We leant more about the bioreactor and its components.
Wrap equipments with aluminum foil to prevent condensation after autoclave.
Media for Seed-Culture and Scale-up Fermentation
1. 2.0litre of distilled water was added to 50g of LB (Luria-Bertani Medium)
2. Once media was prepared, it was autoclaved for 20 minutes.
3. Ampicillin and arabinose was added after the media was cooled to below 50°C.
4. The pH electrode was calibrated using standard buffer solution.
5. The pH probe, pO2 probe, foam, level probe, exhaust condensers, air inlet and exhaust filters were installed.
6. Escherichia coli containing the Green Fluorescent Protein were streaked onto an LB plate.
Answers to further questions:
Differences between microbial bioreactor and mammalian cell bioreactor:
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