Overall objective – To scale up production of green fluorescent protein.
Experiment no.1 Familiarization with the bioreactor and its operation
To familiarize with the protocols for handling the bioreactor
Experiment no. 2 Media and Seed Culture Preparation
To prepare the bioreactor
To prepare for seeding the culture
To scale-up fermentation of culture
Experiment no. 3 Inoculation, fermentation and monitoring
To increase yield of desired protein product (green fluorescent protein)
To monitor cell growth and product formation by manual sampling and measuring absorbance and computer data logging of environment factors in the fermentor
Experiment no. 4 Isolation and purification of product
To harvest the product from the fermentor
To isolate and purify the product GFP(Green fluorescent protein) from the harvest
Experiment no.1 Familiarization with the bioreactor and its operation
To familiarize with the protocols for handling the bioreactor
Experiment no. 2 Media and Seed Culture Preparation
To prepare the bioreactor
To prepare for seeding the culture
To scale-up fermentation of culture
Experiment no. 3 Inoculation, fermentation and monitoring
To increase yield of desired protein product (green fluorescent protein)
To monitor cell growth and product formation by manual sampling and measuring absorbance and computer data logging of environment factors in the fermentor
Experiment no. 4 Isolation and purification of product
To harvest the product from the fermentor
To isolate and purify the product GFP(Green fluorescent protein) from the harvest
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